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Wednesday, July 29, 2020 | History

2 edition of MUC1 expression in human endometrium. found in the catalog.

MUC1 expression in human endometrium.

Neil Anthony Hey

MUC1 expression in human endometrium.

by Neil Anthony Hey

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  • 38 Currently reading

Published by University of Manchester in Manchester .
Written in English


Edition Notes

Thesis (Ph.D) - University of Manchester, Faculty of Medicine.

ContributionsUniversity of Manchester. Faculty of Medicine.
The Physical Object
Pagination306p.
Number of Pages306
ID Numbers
Open LibraryOL21068966M

  Objective To study expression of integrin and epidermal growth factor in sterile patients during endometrial implantation window and discuss the relationship between the integrin and epidermal growth factor and endometrial receptivity. Methods: The research objects were divided into 5 groups with 30 women at the normal gestation age, 30 women exhibiting ovulation following PCOS ovulation. The objective of this study was to investigate the expression of human MUC genes (MUC1, MUC2, MUC5B, MUC5AC and MUC8) in human endometrium and cervix, and to compare and quantitate the expression.

Hey NA, Graham RA, Seif MW, Aplin JD () The polymorphic epithelial mucin MUC1 in human endometrium is regulated with maximal expression in the implantation phase. J Clin Endocrin Metabol – CrossRef Google Scholar. Aplin JD, Hey NA, Graham RA: Human endometrial MUC1 carries keratan sulfate: characteristic glycoforms in the luminal epithelium at receptivity. Glycobiology. ; 8 (3): –

The endometrium, which is of crucial importance for reproduction, undergoes dynamic cyclic tissue remodeling. Knowledge of its molecular and cellular regulation is poor, primarily owing to a lack of study models. Here, we have established a novel and promising organoid model from both mouse and human endometrium. Dissociated endometrial tissue, embedded in Matrigel under WNT-activating.   Reactive gastropathy associated with loss of MUC1 expression in 67% of cases, may play a role in development of its serrated profile (Arch Pathol Lab Med ;) Clinical features Highly expressed by most adenocarcinomas, associated with poor prognosis (Hum Pathol ;).


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MUC1 expression in human endometrium by Neil Anthony Hey Download PDF EPUB FB2

Therefore, reduction of MUC1 expression is observed in uterine lumenal epithelia in many species (Carson et al.

But MUC1 expression persists in the human uterus during the proposed receptive phase (Aplin et al. ; Horne et al. It is possible that MUC1 may function differently in human implantation than in other by:   MUC1 expression has been extensively studied in human endometrium (Aplin et al.,; DeLoia et al., ; Horne et al., ; Wang et al., ); however, there is limited information available on MUC4 and MUC16 expression in human uterineCited by:   MUC1 expression in human endometrium has been investigated using antibodies that recognize peptide epitopes within the tandem repeats (Hey et al.,).

Antibodies including HMFG1, HMFG2, BC2, BC3 and D4, vary in their ability to detect glycoforms of the mucin, which show time and region-specific expression (Aplin et al., Cited by: Human MUC1 expression was detected on Day 1 (Fig.

3, J and L) and also was detected on Day postcoitum (Fig. 3, N and P) in luminal and glandular epithelia (Fig. 3, R and T). Collectively, these data confirm that human MUC1 expression was restricted to endometrial epithelia during the MUC1 expression in human endometrium.

book by: Expression of MUC1 (ADMCKD, ADMCKD1, CD, MCD, MCKD, MCKD1, PEM, PUM) in endometrium tissue. Antibody staining with HPA, HPA, HPA, CAB and CAB in immunohistochemistry.

We use cookies to enhance the usability of our website. MUC1 is a cell-surface and secretory product of endometrial epithelium. Immunohistoehemical studies carried out using two different antibodies to the mucin-type tandem repeat region of MUC1 indicate a cell-surface location in proliferative phase glands, with intracellular deposits accumulating in the early secretory phase.

The MUC1 and MUC20 expression levels in whole endometrial biopsies were determined in 10 paired LH+2 and LH+8 endometrial samples. Cell-type-specific expression pattern was determined in 17 out of the 26 paired (LH+2 and LH+8) endometrial epithelial and stromal cell samples isolated by FACS method described previously (Krjutškov et al., ).

Igarashi TM, Bruner-Tran KL, Yeaman GR, Lessey BA, Edwards DP, Eisenberg E, et al. Reduced expression of progesterone receptor-B in the endometrium of women with endometriosis and in cocultures of endometrial cells exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin.

MUC1 structure and expression. The MUC1 gene encodes a type-I transmembrane glycoprotein that is expressed on the apical surface of most simple epithelia, including mammary gland, female reproductive tract, lung, kidney, stomach, gall bladder, and pancreas as well as some non-epithelial cell types [reviewed in []].The human MUC1 gene spans 4 to 7 kb and is comprised of 7.

Expression of a transmembrane mucin MUC1 is emphasized in most cases of carcinoma. High expression of MUC1 is closely associated with cancer progression and metastasis, leading to poor prognosis.

However, little is known about how MUC1 is overexpressed in malignant tumor. Graham RA, Li T-C, Cooke ID, Aplin JD () Keratan sulphate as a secretory product of human endometrium: cyclic expression in normal women. Hum Reprod – PubMed Google Scholar Hey NA, Aplin JD () Sialyl Lewis x and Sialyl Lewis a are expressed by human endometrial MUC1.

In some studies, expression of endometrial MUC1 has been found to be up-regulated during the peri-implantation period of natural human cyc Expression of MUC1 messenger RNA (mRNA) increases from the proliferative to midsecretory phases and decreases during the late secretory phase (36), which makes the role of reduced MUC1 expression.

Cyclical change of endometrial MUC1 expression in animal models has been extensively documented, and may play an important role in determining the receptivity of endometrium to embryo implantation. Recently, progesterone dependent regulation of MUC1 has also been demonstrated in human endometrium, although the effect was the opposite to that.

Mucin gene expression in the equine endometrium was in broad agreement with that of other species. In the human endometrium, MUC1, MUC2, MUC4, MUC5AC, MUC6, MUC8, and MUC16 are expressed at varying levels and different locations (Gipson et al.,Hebbar et al.,Koscinski et al.,Gipson et al., ).

Mucin 1 (MUC1) is a glycoprotein in human endometrium and is abundant at the luminal epithelial surface in the receptive phase. It has a highly glycosylated ecto-domain that contains keratan. Therefore, in this study, we revisited the global gene expression profile of human endometrial biopsies by using a larger series of patients (n = 31) and samples (n = 62) that were collected during the early secretory phase, 2 days after the LH surge (LH + 2) and the mid-secretory phase (LH +.

In this study, we investigated the expression of mucin 1 (MUC1) mRNA and protein in sheep endometrium at different time points during follicular and luteal phases of estrous cycle, and also determined the effect of steroid hormone treatments and interferon tau (IFNτ) on MUC1 mRNA expression in endometrial cell culture in experiment one, 15 Welsh mountain ewes were.

Expression of the transmembrane mucins, MUC1, MUC4 and MUC16, in normal endometrium and in endometriosis Article (PDF Available) in Human Reproduction 29(8) June with Reads. Expression of MUC1 (ADMCKD, ADMCKD1, CD, MCD, MCKD, MCKD1, PEM, PUM) in cervix, uterine tissue.

Antibody staining with HPA, HPA, HPA, CAB Abstract. After ovulation, progesterone stimulates a temporally regulated secretory transformation in human endometrial epithelium. Using a combination of immunohistochemistry, and Western and Northern blotting, we demonstrate that 1) the polymorphic epithelial mucin MUC1 is secreted by human endometrial epithelium; 2) low levels of both mRNA and core protein are present in the preovulatory.

Background: Acceptance of uterus and reaction between endometrium and embryo has an important role for implantation.

Muc1, an integral membrane mucin, is expressed on the apical surface of uterine epithelial cells and could have effects on its receptivity. Objective: The aim of this study was to evaluate the changes in Muc1 expression of gravid mouse endometrium with and without.Recent evidence points to an important role for Muc1 in embryo implantation.

In this study, Real-time PCR and immunohistochemistry were used to study mRNA and protein levels at, and between, the attachment sites of the endometrium of 18 and 24 pregnant sows.

The results indicate that Muc1 mRNA expression was higher between attachment sites than at attachment sites during. Of all the mucins known to exist within the human genome, only Mucin-1 (MUC1) and Mucin-6 (MUC6) have been found in the human endometrium. When highly expressed along the endometrial cell surface, MUC1 and MUC6 interfere with cellular adhesion between the embryo and uterine lining due to steric hindrance.